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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Blood group systems and antigens - Transfusion reactions and adverse events - Blood components and storage - Antibody detection and identification |
| Topic 2: Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring - Electrolytes, acid-base balance, and blood gases |
| Topic 3: Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Urine formation and composition |
| Topic 4: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components |
| Topic 5: Hematology | 20-25% | - Hemostasis and coagulation testing - White blood cell morphology and diseases - Routine and automated hematology procedures - Red blood cell physiology and disorders |
| Topic 6: Laboratory Operations | 5-10% | - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance |
| Topic 7: Microbiology | 15-20% | - Mycology, parasitology, and virology - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
If Kappa or Lambda is predominant and CD5 is co-expressed with CD19 (CD19/CD5 dual positive lymphocyte population), and CD23 is expressed, chronic lymphocytic leukemia is a probable diagnosis. CD19 is normally found on normal B cell populations and CD5 is normally found on mature T cell populations.
However, CD5 is present on B cells in B-chronic lymphocytic leukemia or Mantle Cell Lymphoma, both abnormal B cell malignant processes.
CD23 expression is not consistent with Mantel cells as shown on the decision tree on the right.
A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following?
- A. A normal B cell
- B. Mantle cell lymphoma
- C. A mature T cell
- D. Chronic lymphocytic leukemia
Correct Answer: D 🗳️
Measuring the turnaround times of troponin tests from the emergency department would provide valuable information. This data represents circumstances in which patient safety may be compromised if results are delayed and treatment is not started as soon as possible.
A laboratory manager wants to evaluate the timeliness of patient services in order to prevent medical errors caused by delay in treatment. Measuring the turnaround time for which of these analytes would provide the most valuable information?
- A. Troponin tests from the emergency department
- B. Routine glucose tests from patients on medical floors
- C. Lipid panels from the outpatient clinic
Correct Answer: A 🗳️
What is the CORRECT blood-to-anticoagulant ratio for coagulations tests?
- A. 5:1
- B. 4:1
- C. 9:1
- D. 10:1
Correct Answer: C 🗳️
n reporting out Gram stains, one should not go beyond what objective observation will allow. In this case, bacterial cells are observed, which are spherical and gram-positive. Their arrangement in tetrads and clusters might be helpful to the physician, suggesting staphylococci. However, one should stop short of naming staphylococci in an official report, as the large gram-positive cocci in tetrads is also suggestive of Micrococcus species, for which human infections have not been reported.
A Gram stain of the serous exudate is shown in the image. The appropriate report would read:
- A. Gram-positive cocci
- B. Smear positive for bacteria
- C. Gram-positive cocci in tetrads and clusters
- D. Gram-positive cocci suggestive of Staphylococcus species
Correct Answer: C 🗳️
In which of the following laboratory situations is a verbal report permissible?
- A. When preoperative test results are needed by the anesthesiologist
- B. None of these answers is correct.
- C. When the report cannot be found at the nurse's station
- D. When the patient is going directly the physician's office
Correct Answer: B 🗳️
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